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mouse monoclonal anti psd95 antibody (Novus Biologicals)

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Novus Biologicals mouse monoclonal anti psd95 antibody

a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), <t>PSD95</t> (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Mouse Monoclonal Anti Psd95 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+psd+95/PSD-95+Antibody+(6G6-1C9)+-+BSA+Free/bio_rxiv__64898__2025__12__06__692741-228-18-22
Average 94 stars, based on 33 article reviews

mouse monoclonal anti psd95 antibody - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease"

Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

Journal: bioRxiv

doi: 10.64898/2025.12.06.692741

Figure Legend Snippet: a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Techniques Used: Western Blot, Two Tailed Test, Immunofluorescence

a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Figure Legend Snippet: a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Techniques Used: Control, Immunofluorescence, Immunostaining, Fluorescence

Binding Assay:

Article Title: Reparative Effects of Stem Cell Factor and Granulocyte Colony-Stimulating Factor in Aged APP/PS1 Mice
Article Snippet: .. The primary antibodies include mouse anti-β-amyloid (1:1000) (clone 4G8, Biolegend, Dedham, MA, USA, #800701), mouse anti-β-amyloid (1:1000) (clone 6E10, Biolegend, Dedham, MA, USA, #803004), rabbit anti-Iba1 (Ionized calcium binding adaptor molecule 1) (1:400, Wako, Osaka, Japan, #019-19741), goat anti-Iba1 (1:400, Novus Biologicals, CO, USA, #NB100-1028), rabbit anti-P2RY12 (purinergic receptor P2Y, G-protein coupled, 12) (1:500, Brigham and Women’s Hospital, MA, USA), rabbit anti-MAP2 (microtubule associated protein 2) (1:600, Millipore Corporation, MA, USA, #AB5622), mouse anti-PSD-95 (postsynaptic density protein 95) (1:500, Novus Biologicals, CO, USA, #NBP2-12872), sheep anti-TREM2 (triggering receptor expressed on myeloid cells-2) (1:300, R&D Systems, Minneapolis, USA, #AF1729), NOS2 (1:100, Millipore Corporation, MA, USA, #AB5382), IL-4 (1:50, Thermo Fisher Scientific, Waltham, MA, USA, # PA5-25165), NeuN (1:500, Millipore Corporation, MA, USA, #MAB377), GFAP (1:500, Sigma-Aldrich, St. Louis, MO, USA, #G3893), CD68 (1:300, Bio-Rad, Hercules, CA, USA, #MCA1957GA), TMEM119 (1:1000, Abcam, Cambridge, MA, #ab209064), and AT8 (1:400, Thermo Fisher Scientific, Waltham, MA, USA, #MN1020). .. After being washed with PBS (3 × 10 min), the sections were incubated with Alexa Fluor 488-, 594- or 647-conjugated donkey anti-mouse, rabbit, goat or sheep secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA) for 2 h at room temperature in the dark.

Article Title: Reparative Effects of Stem Cell Factor and Granulocyte Colony-Stimulating Factor in Aged APP/PS1 Mice
Article Snippet: .. The primary antibodies include mouse anti-β-amyloid (1:1000) (clone 4G8, Biolegend, Dedham, MA, USA, #800701), mouse anti-β-amyloid (1:1000) (clone 6E10, Biolegend, Dedham, MA, USA, #803004), rabbit antiIba1 (Ionized calcium binding adaptor molecule 1) (1:400, Wako, Osaka, Japan, #019-19741), goat anti-Iba1 (1:400, Novus Biologicals, CO, USA, #NB100-1028), rabbit anti-P2RY12 (purinergic receptor P2Y, G-protein coupled, 12) (1:500, Brigham and Women’s Hospital, MA, USA), rabbit anti-MAP2 (microtubule associated protein 2) (1:600, Millipore Corporation, MA, USA, #AB5622), mouse anti-PSD-95 (postsynaptic density protein 95) (1:500, Novus Biologicals, CO, USA, #NBP212872), sheep anti-TREM2 (triggering receptor expressed on myeloid cells-2) (1:300, R&D Systems, Minneapolis, USA, #AF1729), NOS2 (1:100, Millipore Corporation, MA, USA, #AB5382), IL-4 (1:50, Thermo Fisher Scientific, Waltham, MA, USA, # PA5-25165), NeuN (1:500, Millipore Corporation, MA, USA, #MAB377), GFAP (1:500, Sigma-Aldrich, St. Louis, MO, USA, #G3893), CD68 (1:300, Bio-Rad, Hercules, CA, USA, #MCA1957GA), TMEM119 (1:1000, Abcam, Cambridge, MA, #ab209064), and AT8 (1:400, Thermo Fisher Scientific, Waltham, MA, USA, #MN1020). .. After being washed with PBS (3 × 10 min), the sections were incubated with Alexa Fluor 488-, 594- or 647-conjugated donkey anti-mouse, rabbit, goat or sheep secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA) for 2 h at room temperature in the dark.

Image Search Results

Journal: bioRxiv

Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

doi: 10.64898/2025.12.06.692741

Figure Lengend Snippet: a , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in entorhinal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. b–e , Quantification of total tau, AT8, AT270, and pSer396-tau levels from a . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. f , Immunoblot analysis of PARIS, pSer202/pThr205-tau (AT8), pThr181-tau (AT270), pSer396-tau, total tau, and β-actin in frontal cortex samples from 10-month-old mice. The location of PARIS is indicated by arrows. g–j , Quantification of total tau, AT8, AT270, and pSer396-tau levels from f . Data are mean ± SEM (PS19, n = 8; PS19; Paris -/- , n = 5). Statistical significance was assessed by two-tailed t -test. k , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of 10-month-old mice. Scale bar, 50 µm. l , m , Quantification of SYNAPSIN I–positive area and PSD95–positive area from k . Data are mean ± SEM (WT, n = 29; PS19, n = 31; PS19; Paris -/- , n = 29; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Article Snippet: Primary antibodies and working dilutions used were as follows: rabbit polyclonal anti-Synapsin I antibody (Millipore Sigma, AB1543, 1:1000); mouse monoclonal anti-PSD95 antibody (Novus Biologicals, NB300-556, 1:1000); mouse monoclonal anti-STAT3 antibody (Cell Signaling, 9139, 1:1,000); rabbit monoclonal phospho-STAT3 (Tyr705) antibody (Cell Signaling, Cat# 9145, 1:1,000); mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Millipore Sigma, G3893, 1:2,000); rabbit polyclonal anti-Iba1 antibody (FUJIFILM Wako Pure Chemical Corporation, 019-19741, 1:1,000); rabbit polyclonal anti-complement C3 antibody (Invitrogen, PA5-21349, 1:1,000); rat monoclonal anti-CD16/CD32 antibody (Invitrogen, 14-0161-82, 1:1,000); mouse monoclonal anti-β-Amyloid antibody (6E10) (BioLegend, 803001, 1:1,000).

Techniques: Western Blot, Two Tailed Test, Immunofluorescence

Journal: bioRxiv

Article Title: Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease

doi: 10.64898/2025.12.06.692741

Figure Lengend Snippet: a , Percentage of time in the novel arm, total distance traveled, and arm entries in the first Y-maze test. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 9; CamK-PARIS (+Napabucasin), n = 10). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. b , Mean speed during Barnes maze training. Data are mean ± SEM (Control, n = 9; CamK-PARIS, n = 8; CamK-PARIS (+Napabucasin), n = 8). Group differences were assessed by one-way ANOVA followed by Tukey’s post hoc test. c , Representative immunofluorescence images of SYNAPSIN I (green), PSD95 (red), and DAPI (blue) in the hippocampus of mice. Scale bar, 50 µm. d , e , Quantification of SYNAPSIN I–positive area and PSD95–positive area from c . Data are mean ± SEM (Control, n = 33; CamK-PARIS, n = 32; CamK-PARIS (+Napabucasin), n = 31; images from 5–8 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph. f , Representative co-immunostaining images of GFAP (red), C3 (green), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. g–i , Quantification of GFAP and relative C3 fluorescence from f . Data are mean ± SEM (Control, n = 38; CamK-PARIS, n = 47; CamK-PARIS (+Napabucasin), n = 51; images from 7–12 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. j , Representative co-immunostaining images of IBA1 (green), CD16/CD32 (red), and DAPI (blue) in the hippocampus of control and CamK-PARIS mice. Scale bar, 50 µm. k , l , Quantification of IBA1 and relative CD16/CD32 fluorescence from j . Data are mean ± SEM (Control, n = 43; CamK-PARIS, n = 45; CamK-PARIS (+Napabucasin), n = 50; images from 7–10 sections per mouse and 5 mice per group). Statistical significance was assessed by one-way ANOVA followed by Tukey’s post hoc test. Comparisons with P ≤ 0.05 are marked on the graph.

Techniques: Control, Immunofluorescence, Immunostaining, Fluorescence

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mouse monoclonal anti psd95 antibody (Novus Biologicals)

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1) Product Images from "Neuronal PARIS-STAT3 axis drives tau pathology and glial activation in Alzheimer’s disease"

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